First year exam: Biochemistry I

Solution Structure of the oligomerization domain in Dmp53 Original PDB

Structure and Function of Biological Macromolecules

Examination board: Proff. Melino S, Caccuri A, Bottini M, Di Venere A

Examination modality: oral

Topics covered by the examination:

(a) Properties of important biological macromolecules (as carbohydrates, fatty acids, proteins)
(b) Structure and function of metalloproteins, with reference to enzymes involved in the process of cell detoxification (as superoxide dismutase)
(c) Structure and function of hemoproteins, particularly myoglobin and hemoglobin. Gene organization and evolution aspects;
(d) Structure and function of hormones, particularly beta-endorphynes
(e) A spectroscopic study of specific proteins’ active site
(f) Studies of protein structure by spectroscopic techniques
(g) Enzyme kinetics

Second year exam: Biochemistry II

Glycolisis (image generated using BioRender.com)

Metabolism and its regulation

Examination board: Proff. Fezza F, Amelio I, Catani MV, Di Venere A, Gasperi V

Examination modality: short written assay

Topics covered by the examination:

(a) Principles of Bioenergetics, biosynthesis of ATP
(b) Glycolysis
(c) Gluconeogenesis
(d) The pentose phosphate pathway
(e) Catabolism and biosynthesis of fatty acids
(f) Cholesterol metabolism
(g) Amino acid metabolism
(h) The urea cycle
(i) Oxidative phosphorylation
(j) Metabolism of nitrogen bases
(k) Heme biosynthesis

Third year exam: Molecular Biology

Gene expression (image generated using BioRender.com)

Gene expression and its regulation

Examination board: Proff. Gambacurta A, Bernassola F, Agostini M, Piro MC

Examination modality: oral

Topics covered by the examination:

  1. The physical structure of the genome
  2. mRNA stability and localization.
  3. Regulatory non-coding RNA: microRNA, long non-coding RNA e circular-RNA.
  4. Epigenetics and human diseases
  5. Epitranscriptomics
  6. Genome Editing and potential therapeutic application. 
  7. Genomics, epigenomics and transcriptomics of cancer
  8. Interaction between macromolecules: Mechanisms and physiological role

Teaching materials:

  1. Lewin’s Genes XII
  2. Molecular Biology of the Gene VIII Edition
  3. Reviews